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In the HEK293 background, disruption of HASPIN provides a compelling model to dissect the molecular mechanisms underlying chromosome segregation and mitotic checkpoint control. This phosphorylation event is controlled by upstream regulators including the CDK1/cyclin B complex and PLK1, and it creates a docking site for the chromosome passenger complex (CPC) at centromeres. HASPIN encodes a serine/threonine kinase that specifically phosphorylates histone H3 at threonine 3 (H3T3ph) during mitosis, an essential step in chromosome alignment and segregation. Their widespread use in signal transduction, drug discovery, and gene function analysis makes them an ideal host for studying mitotic regulators such as HASPIN.

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